Protein scaffold regeneration free#
Despite being available in various forms and size, and free of morbidity issues, the allografts can cause infectious disease transmission and immunological rejection. On the other side, ~34% of the bone substitutes have allogenic origin (often derived from cadavers). Accounting for ~58% of bone substitutes, autografts remain the gold standard for small defect reconstruction, but are also associated with a number of drawbacks, like infections, bleeding, limited amount of donor bone tissue, the need for a second surgery site for bone graft harvest, donor site morbidity, and chronic pain. On one side, thanks to the presence of growth factors, bone material, and osteogenic cells, autologous grafts have excellent osteoinductive and osteoconductive features. Beyond the implantation of metallic prostheses, bone allo- or auto-grafts are indeed the main available therapeutic solutions for large bone defects ( Laurencin et al., 2006 Calori et al., 2011). Such critical size bone defects are a clinical problem affecting millions of people worldwide, such that autologous bone graft is the second most commonly transplanted tissue after blood. Especially, when the defect size overcomes the healing capacity, a surgical intervention is needed. However, in case of bone tumor removal, traumas with extensive defects or infections, the bone repair process can be impaired such that the damaged area cannot fully and spontaneously regenerate. Bone is constantly remodeled in physiological conditions and presents a strong regenerative capacity to react to fractures. While providing weight-bearing sustainment and assisting locomotion, the 206 bones in the adult human body have other defined biological roles, such as: generation of blood cells (haematopoiesis), physical protection of vital organs like brain or heart, and storage of minerals and growth factors ( Clarke, 2008). The collection of studies herein considered confirms that advances in natural polymer research will be determinant in designing translatable materials for efficient tissue regeneration with forthcoming impact expected in the treatment of bone defects.īone is an essential and multifunctional organ. The regeneration outcomes achieved in vitro and in vivo when the scaffolds are enriched with different cell types, as well as the preliminary clinical applications are presented, before the prospects in this research field are finally discussed. Their combination to other classes of materials (such as micro and nanomaterials) and other innovative strategies to reproduce physiological bone microenvironments in a more faithful way are also illustrated. The present review offers an overview of the various types of natural polymers currently adopted in bone tissue engineering, describing their manufacturing techniques and procedures of functionalization with active biomolecules, and listing the advantages and disadvantages in their respective use in order to critically compare their actual applicability potential. Their assembly and further engineering give rise to a wide plethora of advanced supporting materials, accounting for systems based on hydrogels or scaffolds with either fibrous or porous architecture. As compared to synthetic ones, polymers of natural origin generally present superior biocompatibility and bioactivity. By virtue of their structural versatility, polymers have a predominant role in generating the biodegradable matrices that hold the cells in situ to sustain the growth of new tissue until integration into the transplantation area (i.e., scaffolds). Through the production of substituting materials mimicking the physical and biological properties of the healthy tissue, tissue engineering strategies address an urgent clinical need for therapeutic alternatives to bone autografts. 2Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerlandĭespite considerable advances in microsurgical techniques over the past decades, bone tissue remains a challenging arena to obtain a satisfying functional and structural restoration after damage.1Department of Biomedical Engineering, University of Basel, Basel, Switzerland.Miriam Filippi 1,2, Gordian Born 1, Mansoor Chaaban 2 and Arnaud Scherberich 1,2 *
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